User manual LEICA CRYOEMBEDDING BROCHURE

[. . . ] Precision Cryoembedding System Frozen Sections Become a Work of Art! Precision Cryoembedding System Easily achieve proper specimen orientation and perfect embedding with the unique Precision Cryoembedding System. [. . . ] This technique is perfect for flimsy, tubular, curled, or angular specimens and it is particularly useful for margin resections. 1 Technique 1. Cut the embedded block into pieces on the cold cutting board, keeping pieces cold on the adjacent metal surface. (The main photo, #5 to the left, shows the central section while the inset shows a longitudinal margin. ) 6. Place the cut pieces face down (pieces are face up in the inset photo #6) in the embedding well and freeze using the procedure described under Face Down Cryoembedding. 2 3 4 5 6 The Results A: Trimmed block B: Stained section on slide C: Photomicrograph (sections repositioned in photograph) A B C Paper Cryoembedding Proper Positioning of Difficult Specimens This technique is used to maintain the orientation of delicate or flimsy specimens or to arrange multiple specimens so they will remain in the same plane for sectioning. Technique 1. Soak a small piece of lens paper in embedding medium and flatten it to the dispensing slide. Allow an end of the paper to overhang the edge of the dispensing slide. Touch the lens paper to the cold floor of the embedding well and gently pull the dispensing slide away. Trim through the paper on the trimming portion of the blade and then move to a clean, sharp portion to section the specimen. [. . . ] Our product range includes instruments, systems and consumables for tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers. Order No. [. . . ]