User manual LEICA DMI6000 B BROCHURE

[. . . ] It is capable of effortlessly maintaining the focus under live cell imaging conditions over extended time periods. However, even the most robust microscope is susceptible to sudden temperature fluctuations, for instance if a climate chamber needs to be opened to add a solution to the sample during the course of an experiment. This can result in unsharp images in the time series, or even losing the sample out of focus completely. Explore Life 2 Leica Microsystems has developed the DMI6000 B with Adaptive Focus Control (AFC) for researchers who demand consistent multidimensional imaging without loss of focus. [. . . ] Now, with the addition of the AFC, the microscope becomes the ultimate tool for live cell applications that demand fast reactions to changing conditions. Based on pioneering technology, the AFC principle involves the reflection of a light beam at a suitable surface to keep the distance between the objective and specimen constant. Smoother integration To be truly effective for a wide range of experiments, Adaptive Focus Control is integrated into Leica Microsystems' intuitive software workflow. For full flexibility, AFC can also be operated in stand-alone mode without PC connection using the microscope's function keys. Remote control is possible via the Leica SmartMove or Leica STP6000 control panel. The underlying technique works for all selected contrast methods and does not require taking additional images of the specimen. This ensures that cells remain viable for longer, and deliver reliable results over long periods of time. The Intelligent Automation of the Leica DMI6000 B offers ultimate ease of use. A single push of the `hold focus' button is all it takes ­ the system works automatically in the background, optimizing the results for each objective. The system focuses on the specimen, while you focus on getting results. 140min TIRF imaging with simultaneous AFC MDCK cells expressing GFP-p75 Courtesy of Prof. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany. AFC switched off 0s 30 s 60 s 90 s 120 s 150 s COS cells were transfected with a Golgi specific GFP variant. Shortly after starting the acquisition, 1 ml of an ice-cold salt solution (PBS) was added. Focus drift can be clearly observed when the cold solution is added (60 s). Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany. 4 The need for speed When moving from position to position within a specimen, small differences in focus are often observed as you look down the eyepieces. The impressive speed of the AFC is clearly demonstrated here, as it automatically corrects for these small focus differences in real time. The challenge here is to keep the cells in focus while acquiring high-speed z-stacks. [. . . ] Leica DMI6000 B with Adaptive Focus Control ­ your reliable system solution for live cell imaging. Time saving and improving results. "AFC is such a powerful tool for time lapse experiments. Jacob University Marburg, Department of Clinical Cytobiology and Cytopathology, Marburg, Germany For best publishable results: Building on the proven stability of the Leica DMI6000 B, Adaptive Focus Control keeps the specimen actively in focus ­ whenever and wherever the experiment requires it. Uncompromising speed Utmost compatibility Unrivaled ease of use in All Dimensions 0h 2h 4h 6h 8h 10 h Time series with DIC contrast method MDCK cells (Madin-Darby Canin Kidney) were imaged over a time period of 10 hours. [. . . ]